Journal: The Journal of Physiological Sciences : JPS

Article Title: Changes in adrenoceptor expression level contribute to the cellular plasticity of glioblastoma cells

doi: 10.1016/j.jphyss.2025.100016

Figure Lengend Snippet: Glioblastomas alter their adrenoceptor expression levels depending on their stage of differentiation. (A). Number of RNA sequence reads representing the expression of mRNAs encoding adrenoceptors in GSCs and DGCs of MGG4, MGG6, and MGG8. ADRA1D , ADRA2C , ADRB1 , and ADRB2 , which are well expressed and particularly variable, are highlighted with orange lines. (B). Graph showing the relative expression levels of ADRA1D , ADRA2C , ADRB1 , and ADRB2 in GSCs and DGCs of MGG4, MGG6, and MGG8 as a ratio of DGCs to GSCs. The vertical axis is shown in logarithm; of the four genes, those representing a common pattern of variation in the three cell lines are highlighted with green lines. (C). Western blotting results evaluating protein expression of ADRA1D, ADRB1, and ADRB2 in GSCs and DGCs of MGG4, MGG8, MGG18, and MGG23. (D). Immunostaining images showing changes in ADRA1D expression in GSCs and DGCs of MGG8. (E). Immunostaining image showing ADRA1D expression in tumor tissue of orthotopic xenografts composed of 8GSC-RFP and 8DGC-GFP. ADRA1D, which is upregulated in the differentiated state, is positive in some RFP-positive cells (formerly GSC cells) and negative in some GFP-positive cells (formerly DGC cells), indicating that, like undifferentiated and differentiated markers, the expression pattern of ADRA1D is also heterogeneous. Staining for ADRA1D in normal mouse brain tissue is shown as a control.

Article Snippet: ADRB2 Rabbit Poly Ab , 1:2000 , Proteintech , 13096 −1-AP.

Techniques: Expressing, Sequencing, Western Blot, Immunostaining, Staining, Control

Journal: The Journal of Physiological Sciences : JPS

Article Title: Changes in adrenoceptor expression level contribute to the cellular plasticity of glioblastoma cells

doi: 10.1016/j.jphyss.2025.100016

Figure Lengend Snippet: The antibodies used for Western blotting.

Article Snippet: ADRB2 Rabbit Poly Ab , 1:2000 , Proteintech , 13096 −1-AP.

Techniques: Western Blot, Produced

Journal: Nature Communications

Article Title: Neuromorphic electro-stimulation based on atomically thin semiconductor for damage-free inflammation inhibition

doi: 10.1038/s41467-024-45590-8

Figure Lengend Snippet: a Heatmaps of RNA-sequencing results. R and W represent the 2D ES and non-ES groups, respectively. Adrb2 was significantly enriched compared with the other adrenergic receptor genes. b Volcano plots of candidate DEGs in the microarray datasets based on the screening criteria. Adrb2 showed no change between the non-ES and 2D ES groups. c Schematic of the intersectional genetic strategy used to generate Lyz2-Cre::Adrb2(f/f) mice. The statistical test was Wald test. d The percentage of ADRB2 + CD68 + in the CD68 + cells. These results verified the ablation of ADRB2 on monocytes/macrophages. n = 6 mice; two-sided Student’s unpaired t test; **** p < 0.0001. e ELISA for IL-6 in the non-ES and 2D ES groups of Lyz2-Cre::Adrb2(f/f) and Adrb2(f/f) mice. The decrease of IL-6 was prevented after 2D ES in Lyz2-Cre::Adrb2(f/f) mice. n = 6 mice; two-way ANOVA; F 1,20 = 63.53, p < 0.0001; p ost hoc Tukey test: *** p < 0.001; **** p < 0.0001; ns, p = 0.2134. f Immunostaining of DAPI, IL-6 and CD68. Scale bar: 100 μm. g The percentage of IL-6 + CD68 + cells among CD68 + cells. The results were in accordance with the ELISA data. n = 6 mice; two-way ANOVA; F 1,20 = 58.85, p < 0.0001; post hoc Tukey test: *** p < 0.001; **** p < 0.0001; ns, p = 0.9986. ES: electro-stimulation, non-ES: tendon surgery and flexible electrode implantation without ES group, 2D ES: tendon surgery, flexible electrode implantation and neuromorphic ES based on 2D FGM IDC group, ns: no significance. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Source data are provided as a file.

Article Snippet: The primary antibodies used in this study included the following: rat anti-CD68 (NBP2-33337, Novus Biologicals; 1:400), rabbit anti-IL-6 (ab290735, Abcam; 1:50), rabbit anti-ADRB2 (ab182136, Abcam; 1:100), rabbit anti-Cleaved Caspase-3 (Cat. # 9661 S, CST; 1:400).

Techniques: RNA Sequencing Assay, Microarray, Enzyme-linked Immunosorbent Assay, Immunostaining

Journal: Acta biochimica et biophysica Sinica

Article Title: Saikosaponin D reverses epinephrine- and norepinephrine-induced gemcitabine resistance in intrahepatic cholangiocarcinoma by downregulating ADRB2/glycolysis signaling.

doi: 10.3724/abbs.2023040

Figure Lengend Snippet: Figure 2. Effect of ADRB2 on the proliferation and gemcitabine resistance of iCCA cells (A) Western blot analysis was used to analyze the expression level of ADRB proteins in RBE cells and HuCCT1 cell lines. (B) The efficiency of ADRB2 knockout was determined by real-time quantitative PCR and western blot analysis (***P<0.001 vs NTC). (C) Effects of ADRB2 knockout on NE-stimulated iCCA cell proliferation by CCK8 assay and are presented as a growth curve (***P<0.001 vs norepinephrine+NTC). (D) Effects of ADRB2 knockout on NE-induced gemcitabine resistance in iCCA cells. Data are presented as the mean±SD (n=3).

Article Snippet: Primary antibodies against ADRB2 (#8513), ABCG2 (#42078), MRP1 (#72202), HK2 (#2867), and GLUT1 (#73015) were purchased from Cell Signaling Technology (Danvers, USA).

Techniques: Western Blot, Expressing, Knock-Out, Real-time Polymerase Chain Reaction, CCK-8 Assay

Journal: Acta biochimica et biophysica Sinica

Article Title: Saikosaponin D reverses epinephrine- and norepinephrine-induced gemcitabine resistance in intrahepatic cholangiocarcinoma by downregulating ADRB2/glycolysis signaling.

doi: 10.3724/abbs.2023040

Figure Lengend Snippet: Figure 3. Effects of ADRB2 on aerobic glycolysis and ABC transporter levels in iCCA cells (A,B) Measurement of the extracellular acidification rate (ECAR) in iCCA cells influenced by NE, E and ICI118551 with the XFe24 Extracellular Flux Analyser. (C) Glycolytic variations (glycolysis, glycolytic capacity and glycolytic reserve) in each group were summarized from raw data (**P<0.01, ***P<0.001 vs control; #P<0.05 vs NE). (D) Glucose consumption in each group was detected using a glucose assay kit (***P<0.001 vs control; ###P<0.001 vs NE). (E) Production of lactic acid in each group was assayed using a Lactic acid production detection kit (***P<0.001 vs control; ##P<0.01 vs NE). (F) Western blot analysis was used to analyse the protein expression levels of HK2, GLUT1, ABCG2 and MRP1in RBE cells and HuCCT1 cell lines from each group. Data are presented as the mean±SD (n=3).

Article Snippet: Primary antibodies against ADRB2 (#8513), ABCG2 (#42078), MRP1 (#72202), HK2 (#2867), and GLUT1 (#73015) were purchased from Cell Signaling Technology (Danvers, USA).

Techniques: Control, Glucose Assay, Western Blot, Expressing

Journal: Acta biochimica et biophysica Sinica

Article Title: Saikosaponin D reverses epinephrine- and norepinephrine-induced gemcitabine resistance in intrahepatic cholangiocarcinoma by downregulating ADRB2/glycolysis signaling.

doi: 10.3724/abbs.2023040

Figure Lengend Snippet: Figure 5. Effect of SSD on ADRB2 expression in iCCA cells stimulated with NE The effects of SSD on the levels of ADRB2 were determined by western blot analysis (A) and real-time quantitative PCR (B). Data are presented as the mean±SD (n=3). ***P<0.001 vs NE.

Article Snippet: Primary antibodies against ADRB2 (#8513), ABCG2 (#42078), MRP1 (#72202), HK2 (#2867), and GLUT1 (#73015) were purchased from Cell Signaling Technology (Danvers, USA).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction

Journal: Acta biochimica et biophysica Sinica

Article Title: Saikosaponin D reverses epinephrine- and norepinephrine-induced gemcitabine resistance in intrahepatic cholangiocarcinoma by downregulating ADRB2/glycolysis signaling.

doi: 10.3724/abbs.2023040

Figure Lengend Snippet: Figure 6. Effect of SSD on ADRB2/glycolysis signaling in iCCA cells stimulated with NE (A) Measurement of the extracellular acidification rate (ECAR) in iCCA cells influenced by NE with/without SSD (2.5 and 5 μM) with the XFe24 Extracellular Flux Analyzer. (B) Glycolytic variations (glycolysis, glycolytic capacity and glycolytic reserve) in each group were summarized from raw data (*P<0.05, **P<0.01, ***P<0.001 vs NE). (C) Glucose consumption in each group was detected using a glucose assay kit (**P<0.01, ***P<0.001 vs NE). (D) Production of lactic acid in each group was assayed using a Lactic acid production detection kit (**P<0.01, ***P<0.001 vs NE). (E) Western blot analysis was used to analyse the protein expression levels of HK2, GLUT1, ABCG2 and MRP1 in RBE cells and HuCCT1 cell lines from each group. Data are presented as the mean±SD (n=3).

Article Snippet: Primary antibodies against ADRB2 (#8513), ABCG2 (#42078), MRP1 (#72202), HK2 (#2867), and GLUT1 (#73015) were purchased from Cell Signaling Technology (Danvers, USA).

Techniques: Glucose Assay, Western Blot, Expressing